Necropsies & Histopathology

For necropsies, live animals are preferred. RRDA offers Basic, General, and Comprehensive Protocols:

Basic Protocol (Necropsy Alone):

• Physical examination
• Euthanasia
• Ectoparasite exam
• Enteric parasite exam
  Bacterial screening for respiratory and enteric pathogens (Bordetella bronchiseptica, Streptococcus pneumoniae, Klebsiella pneumoniae, Salmonella spp. and any requested bacteria)
• Gross dissection and histopathology of any lesioned organs (up to 3 tissues)
• No serology

While a Gross Necropsy alone is offered, it provides very little information, as no histopathology is performed even if lesions are observed. However, upon request, tissues can be collected and held for one month for possible histopathology.

General Protocol:

• Physical examination
• Blood Collection
• Euthanasia
• Ectoparasite exam
• Enteric parasite exam
  Bacterial screening for respiratory and enteric pathogens (Bordetella bronchiseptica, Streptococcus pneumoniae, Klebsiella pneumoniae, Salmonella spp. and any requested bacteria)
• Gross dissection, tissue collection, fixation and processing any lesioned organs (up to three organs)
• General Serology Panel: MHV, Sendai, PVM, MVM, MPV, MNV, M. pulmonis, GD-VII, and EDIM).

Comprehensive Protocol:

• Physical examination
• Blood Collection
• Euthanasia
• Ectoparasite exam
• Enteric parasite exam: Bacterial screening for respiratory and enteric pathogens (Bordetella bronchiseptica, Streptococcus pneumoniae, Klebsiella pneumoniae, Salmonella spp. and any requested bacteria)
• Gross dissection, tissue collection, fixation and processing of major organs (lung, liver, spleen, kidneys, salivary glands, GI tract) and any lesioned organs
• Histopathology evaluations of H&E slide preparation and interpretation of major organs (lung, liver, spleen, kidneys, salivary glands, GI tract) and any lesioned organs
• Histopathology evaluation of other organs such as brain, skeletal muscle and skin are available upon request.
• Advanced Serology Panel: MHV, Sendai, PVM, MVM, MNV, MPV, M. pulmonis, GD-VII, EDIM, REO-3, LCMV, ECTRO, NS-1.

Major Organ Panel
In addition to the above protocols, the Major Organ Panel is available. This includes a necropsy and histopathology of the lungs, heart, liver, kidneys, and spleen.

The Complete Diagnostic Panel includes a necropsy and histopathology of the lungs, heart, liver, kidneys, spleen, brain, salivary gland, stomach, duodenum, jejunum, ileum, cecum and colon.

RESEARCH SERVICES :
Experimental Pathology Services and Immunohistochemistry services are available on request for submitted tissues and/or slides such as frozen or paraffin sections, for immunostaining, in situ hybridization, and detection of gene expression in cell lines and/or tissues.