PATHOLOGY AND FOLLOW-ON STUDIES

Once the tissues have been collected, there are a variety of follow - on studies that can be performed. Our capabilities include:

• Basic histology (H&E strain)
• Use of special stains (Masson Trichrome, etc.)
• Proliferation and apoptosis immunostains
• Immunohistochemistry for both monoclonal and polyclonal antibodies
• Fluorescent immunohistochemistry
• Cellular hypoxia measurements
• Blood vessel density measurements
• Biochemical assays for target effectiveness on treated animal tissues
• Molecular biology is an integral tool for analyzing treated tissues, building molecular probes,
• and developing new animal models. Some of our expertise includes
• DNA, RNA, and protein extraction from cell and tissue l Western, Northern, and Southern blot analysis, immunoprecipitation and kinase assays
• Gene construction, transfection, and expression
• DNA sequencing and mutation detection
• cDNA synthesis, primer design, PCR, and RT-PCR
• Genotyping, DNA enzymology, plasmid DNA sub-cloning
• Developing knockout mouse and transgenic mice.