Test |
Minimum (ul) |
Pipetted (ul) |
Preferred (ul) |
Acceptable |
Special Requirements |
Alkaline Phosphatase Isoenzymes |
100 |
25 |
500 |
|
|
Angiotensin Converting Enzyme (ACE) |
100 |
25 |
200 |
|
|
Apolipoprotein |
100 |
25 |
200 |
|
|
Bile Acids |
100 |
20 |
200 |
|
|
CK Isoenzymes |
250 |
|
500uL |
|
|
Cholinesterase |
100 |
25 |
200 |
500uL of EDTA whole blood |
|
CH50 Total Complement |
100 |
25 |
200 |
|
|
C3 Complement |
100 |
25 |
200 |
|
|
C4 Complement |
100 |
25 |
200 |
|
|
Ferritin |
100 |
25 |
200 |
|
|
Free Fatty Acids |
100 |
25 |
200 |
|
|
Free Hemoglobin |
100 |
25 |
200 |
Heparinized Plasma |
No vacuum tubes, use syringe. |
HDL Cholesterol |
100 |
25 |
200 |
|
|
Haptoglobin |
100 |
50 |
200 |
|
|
Immunoglobin A |
100 |
25 |
200 |
|
|
Immunoglobin G |
100 |
25 |
200 |
|
|
Immunoglobin M |
100 |
25 |
200 |
|
|
Iron |
100 |
12 |
200 |
|
|
LD Isoenzymes |
100 |
25 |
500 |
|
Room temperature, ship immediately, do not freeze |
LDL Cholesterol |
100 |
25 |
200 |
|
|
|
|
|
|
|
|
Lactic Acid |
100 |
25 |
200uL |
Fluoride Plasma |
Whole blood - Prepare 1 part blood and 1 part 8% perchloric acid precipitate. Free supernatant. |
Myoglobin |
100 |
25 |
200 |
|
|
NAG |
100 |
25 |
200 urine |
|
unpreserved |
5'-Nucleotidase |
100 |
5 |
200 |
|
|
Phospholipids |
100 |
25 |
|
|
|
Protein Electrophoresis |
100 |
25 |
500uL |
|
|
Sorbitol Dehyrdrogenase (SDH) |
100 |
25 |
200 |
|
|
Unsaturated Iron Binding Capacity |
100 |
8 |
|
|
|
Test |
Minimum (ul) |
Pipetted (ul) |
Preferred (ul) |
Acceptable |
Special Requirements |
Adrenocorticotropic Hormone (ACTH) |
100 |
25 |
500 |
|
Collect in cold siliconized tube, separate and freeze immediately. |
Aldosterone |
150 |
100 |
500 |
|
|
Antidiuretic Hormone (ADH) |
500 |
400 |
750 |
|
|
Calcitonin |
250 |
200 |
500 |
|
|
Catacholamines |
250 |
200 |
500 |
|
|
Corticosterone |
150 |
50 |
200 |
|
|
Cortisol |
120 |
10 |
200 |
|
|
DHEA |
150 |
100 |
500 |
|
|
DHEA Sulfate |
100 |
25 |
500 |
|
|
Dihydrotestosterone |
150 |
50 |
200 |
|
|
Estradiol |
300 |
250 |
500 |
|
|
Folic Acid |
250 |
200 |
500 |
Heparinized Plasma |
For Whole Blood - Record Hematocrit. 100uL of blood in 2mL of 1% ascorbic acid (fresh). |
Test |
Minimum (ul) |
Pipetted (ul) |
Preferred (ul) |
Acceptable |
Special Requirements |
Adrenocorticotropic Hormone (ACTH) |
100 |
25 |
500 |
|
Collect in cold siliconized tube, separate and freeze immediately. |
Follicle Stimulating Hormone (FSH |
150 |
100 |
500 |
|
|
Glucagon |
250 |
|
500 |
|
EDTA with 5000 U Trasylal/10mL of blood |
Growth Hormone |
200 |
100 |
500 |
|
|
Histamine |
100 |
|
250 |
|
EDTA plasma |
Insulin-Like Growth Factor |
50 |
10 |
100 |
|
EDTA or Heparin |
Insulin |
100 |
|
200 |
|
|
Leutenizing Hormone (LH) |
150 |
100 |
500 |
|
|
Leptin |
150 |
100 |
500 |
|
|
N-telopeptide |
75 |
50 |
100 |
|
|
Osteocalcin |
50 |
20 |
100 |
Heparin acceptable |
|
Parathyroid Hormone (PTH-intact) |
150 |
50 |
250 |
|
|
Pro-BNP |
100 |
150 |
200 |
|
|
Progesterone |
25 |
125 |
200 |
|
|
Prolactin |
100 |
200 |
500 |
|
|
Thyroid Stimulating Hormone |
100 |
150 |
500 |
|
|
Testosterone |
20 |
120 |
150 |
|
|
Thyroxine (T4) |
75 |
25 |
125 |
|
|
Free (T4) |
200 |
|
500 |
|
|
Tri-iodothyronine |
125 |
100 |
150 |
|
Heparin acceptable |
T3 Uptake |
200 |
|
500 |
|
|
Troponin I |
50 |
|
175 |
|
|
Test |
Minimum (ul) |
Pipetted (ul) |
Preferred (ul) |
Acceptable |
Special Requirements |
Vitamin B12 |
200 |
200 |
250 |
|
|
Vitamin D 1,25 OH |
25 |
250 |
500 |
|
|
