Individual Chemistry

Test	Minimum (ul)	Pipetted (ul)	Preferred (ul)	Acceptable	Special Requirements
Alkaline Phosphatase Isoenzymes	100	25	500
Angiotensin Converting Enzyme (ACE)	100	25	200
Apolipoprotein	100	25	200
Bile Acids	100	20	200
CK Isoenzymes	250		500
Cholinesterase	100	25	200	500 uL of EDTA whole blood
CH50 Total Complement	100	25	200
C3 Complement	100	25	200
C4 Complement	100	25	200
Ferritin	100	25	200
Free Fatty Acids	100	25	200
Free Hemoglobin	100	25	200	Heparinized plasma	No vacuum tubes, use syringe.
HDL Cholesterol	100	25	200
Haptoglobin	100	50	200
Immunoglobin A	100	25	200
Immunoglobin G	100	25	200
Immunoglobin M	100	25	200
Iron	100	12	200
LD Isoenzymes	100	25	500		Room temperature, ship immediately, do not freeze
LDL Cholesterol	100	25	200

Lactic Acid	100	25	200	Fluoride Plasma	Whole blood – Prepare 1 part blood and 1 part 8% perchloric acid precipitate. Free supernatant.
Myoglobin	100	25	200
NAG	100	25	200 urine		unpreserved
5'-Nucleotidase	100	5	200
Phospholipids	100	25
Protein Electrophoresis	100	25	500
Sorbitol Dehyrdrogenase (SDH)	100	25	200
Unsaturated Iron Binding Capacity	100	8

Immunoassays

Test	Minimum (ul)	Pipetted (ul)	Preferred (ul)	Acceptable	Special Requirements
Adrenocorticotropic Hormone (ACTH)	100	25	500		Collect in cold siliconized tube, separate and freeze immediately.
Aldosterone	150	100	500
Antidiuretic Hormone (ADH)	500	400	750
Calcitonin	250	200	500
Catacholamines	250	200	500
Corticosterone	150	50	200
Cortisol	120	10	200
DHEA	150	100	500
DHEA Sulfate	100	25	500
Dihydrotestosterone	150	50	200
Estradiol	300	250	500
Folic Acid	250	200	500	Heparinized plasma	For whole blood – Record hematocrit. 100uL of blood in 2mL of 1% ascorbic acid (fresh).

Hormones

Test	Minimum (ul)	Pipetted (ul)	Preferred (ul)	Acceptable	Special Requirements
Adrenocorticotropic Hormone (ACTH)	100	25	500		Collect in cold siliconized tube, separate and freeze immediately.
Follicle Stimulating Hormone (FSH	150	100	500
Glucagon	250		500		EDTA with 5000 U Trasylal/10mL of blood
Growth Hormone	200	100	500
Histamine	100		250		EDTA plasma
Insulin-Like Growth Factor	50	10	100		EDTA or heparin
Insulin	100		200
Leutenizing Hormone (LH)	150	100	500
Leptin	150	100	500
N-telopeptide	75	50	100
Osteocalcin	50	20	100	Heparin acceptable
Parathyroid Hormone (PTH-intact)	150	50	250
Pro-BNP	100	150	200
Progesterone	25	125	200
Prolactin	100	200	500
Thyroid Stimulating Hormone	100	150	500
Testosterone	20	120	150
Thyroxine (T4)	75	25	125
Free (T4)	200		500
Tri-iodothyronine	125	100	150		Heparin acceptable
T3 Uptake	200		500
Troponin I	50		175

Vitamins