Test Volume Requirements
Hematology:
The only acceptable sample for a complete blood count (CBC) and reticulocyte count is EDTA whole blood ( lavender top tube ). The Minimum sample size is 150uL ) plus two slides made at the site. Do not freeze the whole blood
Coagulation
Sodium citrate tubes (blue top): Citrate tubes ( Blue Top ) should be filled to the top. For small volumes, sample may be collected in a tube with a ratio of one part sodium citrate and nine parts blood. Gently mix specimen by inverting at least 5-6 times and place immediately at 4°C for up to 30 minutes before being spun in a centrifuge. . Up The following tests can be performed on 500uL of frozen plasma. Minimum sample size for Individual test is 250uL.
- Prothrombin Time (PT)
- Activated Prothrombin Time (APTT)
- Fibrinogen
Sample: Serum or plasma samples (serum is preferred) collected in red top or SST, red/camouflage top tubes. Both plain and SST tubes must be allowed to clot at 4°C for 30 minutes 60 minutes. Then the samples must be centrifuged at 7000 rpm for 10 minutes, and the serum be decanted and frozen at -80°C for clinical chemistry analysis.
Volume: Up to 20 tests can be performed on 500uL. Minimum sample size for Individual test is 150uL.