Rabbit & Rodent Diagnostic Associates

Recommended Sample Size for PCR



Sample

Amount*

Cultured cells

1 x 10^7

Liver

25 mg (~2mm 3)

Brain+

25 mg

Lung+

25 mg

Heart/muscle+

25 mg

Kidney

25 mg

Spleen+

10 mg

Plant leaf

100 mg

Mouse tail

0.8-1.2 cm

Blood

1 ml

Buffy coat

50 ul

DNA (PCR)

500 ng/rxn

RNA (PCR)

500 ng/rxn

DNA (Q-PCR)

50 ng/rxn

RNA (Q-RT-PCR)

100 ng/rxn



+These are difficult tissues and may require special processing.
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