Recommended Sample Size for PCR

Sample	Amount
Cultured cells	1 x 107
Liver	25 mg (~2mm3)
Brain+	25 mg
Lung+	25 mg
Heart/muscle+	25 mg
Kidney	25 mg
Spleen+	10 mg
Plant leaf	100 mg
Mouse tail	0.8-1.2 cm
Blood	1 ml
Buffy coat	50 ul
DNA (PCR)	500 ng/rxn
RNA (PCR)	500 ng/rxn
DNA (Q-PCR)	50 ng/rxn
RNA (Q-RT-PCR)	100 ng/rxn

+These are difficult tissues and may require special processing.