Construction of full-length cDNA libraries
Libraries can be either subtracted, normalized or non-normalized. Libraries can be either cloned into general cloning vector and transformed into bacterial cells or cloned into phage lambda arms and packaged into phage.
- Normalized, full-length or fragmented expression libraries : Un-amplified phage stock in SM (400-500ul containing 10 4 - 10 7 pfu, depending on average insert size).
- Normalized, fragmented, oriented cDNA library (OFCEL): cloned into mammalian expression vector DNA stock of 100ug library plasmids representing ~10 6 independent recombinants
- Shotgun DNA libraries : Bacterial glycerol stock containing independent recombinants - number of recombinant clones depends on initial genome length and on desired average insert size.