Expression Analysis by Quantitative PCR

MDS offers expression analysis assays by Q-PCR in order to monitor changes in expression of particular genes and or cytokines, in response to drug treatment.

The samples can be analyzed using either a relative or absolute standard. A relative standard could be a specific sample that would be used on all plates of the study. An absolute standard could be cDNA made from an in vitro synthesized RNA that contains the target, diluted into an appropriate RNA

Qualification assays, to determine test article interference with assay performance, are performed before the quantitative assays. Each Q-PCR study has multiple assay controls to confirm the optimal performance of the assay and to confirm the absence of reagent contamination and PCR inhibitors, thereby ensuring that reproducibility, sensitivity, and accuracy are achieved.

Expression Assays

• Human P53 gene
• Human PDE7 (phosphodiesterase 7) gene
• Mouse VEGF receptor gene
• Mouse DHFR gene
• Rhesus monkey CFTR gene
• Cytokine

Controls

• GAPDH (mouse, rabbit, rhesus monkey, hamster, human)
• High copy targets (CHO and human)