Isoenzyme Analysis
Isoenyzyme analysis can determine the species identity of your cell line and determine if your cell line has been contaminated by a cell line of a different species. From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.
Standard isoenzyme analyses include d etermination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:
- Glucose - 6 - Phosphate Dehydrogenase (G6PD)
- Lactate Dehydrogenase (LD)
- Malate Dehydrogenase (MD)
- Nucleoside Phosphorylase (NP)
- Aspartate Aminotransferase (AST)
- Peptidase B (PepB)
- G-Protein Coupled Receptors (GPCR)
- cAMP Phosphodiesterase Assay
- Protein Kinase Assay (PK)